PHARMANEST : AN INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACEUTICAL SCIENCES - Volume 4, Issue 6, November - December 2013

Pages: 1123-1130

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QUANTITATIVE ANALYSIS OF CILNIDIPINE AND TELMISARTAN IN TABLETS BY HIGH PERFORMANCE THIN-LAYER CHROMATOGRAPHY WITH ULTRAVIOLET ABSORPTION DENSITOMETRIC DETECTION

Author: M.HARIPRIYA*, Dr. P. JAYASEKHAR

Category: Pharmaceutical Sciences

Abstract:

A novel HPTLC method for the simultaneous estimation of Cilnidipine and Telmisartan in two component dosage forms has been developed and validated. Standard and sample solutions of cilnidipine and telmisartan were applied to precoated silica gel G 60 F254 HPTLC plates and the plates were developed with Toluene: Ethyl acetate: DMF in the ratio 6.5: 3.0: 0.5 (v/v), as mobile phase. UV detection was performed densitometrically at 260 nm. The Rf value for cilnidipine and telmisartan was found to be 0.47and 0.17 respectively. The correlation coefficients 0.9917 and 0.9852 for telmisartan respectively. The calibration curve was found to be linear between 100 to 1200 ng/spot for both cilnidipine and telmisartan. The limits of detection and quantitation were found to be 12.6 and 38.28 ng/spot, respectively for cilnidipine and 61.05 and 185.0 ng/spot, respectively for telmisartan. The results have been validated statistically as per ICH guidelines. The developed method can be successfully employed for the simultaneous determination of cilnidipine and telmisartan in marketed tablet formulation.

Keywords: Cilnidipine, Telmisartan, HPTLC, Validation