PHARMANEST : AN INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACEUTICAL SCIENCES - Volume 4, Issue 6, November - December 2013

Pages: 1438-1446

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DESIGN OF CULTURAL CONDITIONS FOR ENHANCEMENT OF ALKALINE PROTEASE PRODUCTION

Author: SMRITHI, K NARAYANAN, VENKATESH KAMATH B*

Category: Pharmaceutical Sciences

Abstract:

Among the various industrial enzymes studied, proteases are the most widely explored enzyme. They occupy the top position in terms of the amount of enzyme produced commercially. In the present study, soil samples collected from various locations around Manipal, Udupi District, Karnataka were screened for proteolytic activity. Casein agar medium was used as basal medium. The isolate showing highest activity during primary screening was further studied for proteolytic activity. Among the isolates screened, KWF-2 obtained from drains of kitchen waste, showed significant proteolytic activity. Therefore, it was further studied by shake flask culture method. Protease yield was improved through cultural condition optimization. These conditions were optimized by one-factor-at-a-time method. The protease was found to be an alkaline protease. Due to the promising result, further studies on characterization of the enzyme are being carried out.

Keywords: Screening, Protease, Optimization.