PHARMANEST : AN INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACEUTICAL SCIENCES - Volume 4, Issue 6, November - December 2013

Pages: 1479-1491

Print Article   Download XML  Download PDF

QUANTITATIVE ANALYSIS OF SILODOSIN IN CAPSULES USING UV SPECTROPHOTOMETRY AND RP-HPLC METHODS: APPLICATION TO DISSOLUTION TESTING

Author: CH. DEVADASU*, P. RAVISANKAR, P. SRINIVASA BABU, S.GANANADHAMU, S. SOWJANYA

Category: Pharmaceutical Sciences

Abstract:

Assay of Silodosin in capsule formulation was done by two ultraviolet spectrophotometric and one isocratic RP-HPLC method. The wavelength of maximum absorption for Silodosin in water was found at 268nm (method-I).The area under the absorption curve was recorded between 263nm and 273nm for each solution (method-II). A reversed phase column C18 (250 x 4.6 mm, 5µm particle size), mobile phase consisting of phosphate buffer pH 3.2: Acetonitrile (60:40v/v) with a flow rate 1.0 mL/min. was used. The effluents of the column were monitored through a variable wavelength UV detector at 230nm (method-III). The proposed methods obeys linearity in the range of 10-60 µg/mL and 4-20 µg/mL for method-I, II and method-III respectively with correlation coefficient values above 0.999. The % RSD was found to be less than 2.0.The mean recoveries were found in the range of 97.50- 99.75%. Validation of the developed methods was performed in terms of accuracy, precision, linearity, etc. The proposed HPLC method has been applied to dissolution testing of the formulation.

Keywords: Silodosin, Reversed Phase HPLC, Validation, Dissolution testing