PHARMANEST : AN INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACEUTICAL SCIENCES - Volume 4, Issue 6, November - December 2013

Pages: 1569-1581

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STUDIES ON OPTIMIZATION OF ANTIBIOTIC PRODUCTION BY ISOLATE C9

Author: *S. JOSHNA RANI

Category: Pharmaceutical Sciences

Abstract:

A new actinomycete species (Pseudonocardia species) which was designated as C9 with broad antibacterial activities was isolated from soil samples of Tirumala Hills and characterized by our Lab. Nutritional and cultural conditions for the production of antibiotic by this organism under shakeflask conditions have been optimized. The results shown the inhibition rate is the highest under the optimum medium composition containing soyabean meal 2.5%, Glucose 2.6%, NaNO3 0.5%, K2HPO4 0.4%, Nacl 0.25%, ZnSO4 0.004%, CaCO3 0.04%. Maximum antibiotic activity is obtained at the inoculation volume 10%, medium to flask ratio was maintained at 0.2 levels, incubated at 280C on a rotary shaker (140 rpm) fermentation was terminated at 144hr. The results indicated that against this test organism (pseudomonas fluorescens) the zone of inhibition observed in the initial production was 28mm, which increased to 33mm in production medium PM, after optimization of the constituents and cultural conditions of production medium PM the zone of inhibition increased to 36mm. this indicates that a significant increase in antibiotic production was achieved after optimization.

Keywords: Actinomycete, Antibiotics, nutrient effects, cultural conditions.